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pgadt7 rec vector  (TaKaRa)


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    Structured Review

    TaKaRa pgadt7 rec vector
    Pgadt7 Rec Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgadt7+rec/pGADT7+AD+Vector/pm40796552-543-9-11
    Average 99 stars, based on 3966 article reviews
    pgadt7 rec vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Characterization of Various Subunit Combinations of ADP-Glucose Pyrophosphorylase in Duckweed ( Landoltia punctata )
    Article Snippet: The purified PCR products of LpAGPLs were digested with Pci I/ Eco R I (TaKaRa, Shiga, Japan), while pGADT7-Rec was double-digested with Nco I/ Eco R I (TaKaRa, Shiga, Japan). .. After purification, the digestion fragments of LpAGPLs and pGADT7-Rec were ligated by T4 DNA ligase (TaKaRa, Shiga, Japan) after purification and transformed into E. coli DH5 α competent cells via electroporation. ..

    Article Title: Stimulatory effect of peritrophin on vitellogenesis in female banana shrimp, <i>Fenneropenaeus merguiensis</i>
    Article Snippet: Ovarian development is an important physiological process in female organisms.. In shrimp, the focus of this present study, it is controlled by several factors, such as peptide hormones, steroid hormones, and neurotransmitters (Jayasankar et al., 2020; Nagaraju, 2011).. The ovarian development in shrimp can be divided into four main stages; previtellogenic, early vitellogenic, late vitellogenic, and mature stages (Ayub & Ahmed, 2002).

    Transformation Assay:

    Article Title: Characterization of Various Subunit Combinations of ADP-Glucose Pyrophosphorylase in Duckweed ( Landoltia punctata )
    Article Snippet: The purified PCR products of LpAGPLs were digested with Pci I/ Eco R I (TaKaRa, Shiga, Japan), while pGADT7-Rec was double-digested with Nco I/ Eco R I (TaKaRa, Shiga, Japan). .. After purification, the digestion fragments of LpAGPLs and pGADT7-Rec were ligated by T4 DNA ligase (TaKaRa, Shiga, Japan) after purification and transformed into E. coli DH5 α competent cells via electroporation. ..

    Article Title: The necrotrophic effector ToxA from Parastagonospora nodorum interacts with wheat NHL proteins to facilitate Tsn1-mediated necrosis.
    Article Snippet: Combined double-stranded cDNA samples (each containing approximately 300 ng DNA) were used to generate the prey library according to the manufacturer’s recommendations (Make Your Own ‘Mate & Plate’ Library System User Manual; Takara). .. For yeast mating, the bait construct pGBKT7-ToxA was transformed into Y2HGold (Takara) and the prey library constructs in pGADT7-Rec (Takara) were transformed into yeast strain Y187 (Takara). ..

    Electroporation:

    Article Title: Characterization of Various Subunit Combinations of ADP-Glucose Pyrophosphorylase in Duckweed ( Landoltia punctata )
    Article Snippet: The purified PCR products of LpAGPLs were digested with Pci I/ Eco R I (TaKaRa, Shiga, Japan), while pGADT7-Rec was double-digested with Nco I/ Eco R I (TaKaRa, Shiga, Japan). .. After purification, the digestion fragments of LpAGPLs and pGADT7-Rec were ligated by T4 DNA ligase (TaKaRa, Shiga, Japan) after purification and transformed into E. coli DH5 α competent cells via electroporation. ..

    Construct:

    Article Title: The necrotrophic effector ToxA from Parastagonospora nodorum interacts with wheat NHL proteins to facilitate Tsn1-mediated necrosis.
    Article Snippet: Combined double-stranded cDNA samples (each containing approximately 300 ng DNA) were used to generate the prey library according to the manufacturer’s recommendations (Make Your Own ‘Mate & Plate’ Library System User Manual; Takara). .. For yeast mating, the bait construct pGBKT7-ToxA was transformed into Y2HGold (Takara) and the prey library constructs in pGADT7-Rec (Takara) were transformed into yeast strain Y187 (Takara). ..

    Article Title: Graphene oxide enhances aphid resistance in sorghum via the miR319 ‐ SbTCP7 ‐ SbLOX3 Pathway
    Article Snippet: .. The SbTCP7 CDS was integrated into the pGADT7‐Rec (Clontech) vector to construct the prey. ..

    Clone Assay:

    Article Title: The Formin-Homology Protein SmDia Interacts with the Src Kinase SmTK and the GTPase SmRho1 in the Gonads of Schistosoma mansoni
    Article Snippet: .. Finally, cDNA was cloned by a recombination step in frame with the Gal4 activation domain (Gal4 AD) into pGADT7-Rec (prey vector; leucin selection marker LEU; Clontech). ..

    Activation Assay:

    Article Title: The Formin-Homology Protein SmDia Interacts with the Src Kinase SmTK and the GTPase SmRho1 in the Gonads of Schistosoma mansoni
    Article Snippet: .. Finally, cDNA was cloned by a recombination step in frame with the Gal4 activation domain (Gal4 AD) into pGADT7-Rec (prey vector; leucin selection marker LEU; Clontech). ..

    Selection:

    Article Title: The Formin-Homology Protein SmDia Interacts with the Src Kinase SmTK and the GTPase SmRho1 in the Gonads of Schistosoma mansoni
    Article Snippet: .. Finally, cDNA was cloned by a recombination step in frame with the Gal4 activation domain (Gal4 AD) into pGADT7-Rec (prey vector; leucin selection marker LEU; Clontech). ..

    Marker:

    Article Title: The Formin-Homology Protein SmDia Interacts with the Src Kinase SmTK and the GTPase SmRho1 in the Gonads of Schistosoma mansoni
    Article Snippet: .. Finally, cDNA was cloned by a recombination step in frame with the Gal4 activation domain (Gal4 AD) into pGADT7-Rec (prey vector; leucin selection marker LEU; Clontech). ..

    Ligation:

    Article Title: Arabidopsis SEC13B Interacts with Suppressor of Frigida 4 to Repress Flowering
    Article Snippet: .. Then, the products were inserted into pGBKT7 and pGADT7-Rec (Clontech, Shiga, Japan) vectors via restriction endonuclease digestion and ligation. ..

    Article Title: Arabidopsis SEC13B Interacts with Suppressor of Frigida 4 to Repress Flowering.
    Article Snippet: .. Then, the products were inserted into pGBKT7 and pGADT7-Rec (Clontech, Shiga, Japan) vectors via restriction endonuclease digestion and ligation. ..

    Polymerase Chain Reaction:

    Article Title: Stimulatory effect of peritrophin on vitellogenesis in female banana shrimp, <i>Fenneropenaeus merguiensis</i>
    Article Snippet: Ovarian development is an important physiological process in female organisms.. In shrimp, the focus of this present study, it is controlled by several factors, such as peptide hormones, steroid hormones, and neurotransmitters (Jayasankar et al., 2020; Nagaraju, 2011).. The ovarian development in shrimp can be divided into four main stages; previtellogenic, early vitellogenic, late vitellogenic, and mature stages (Ayub & Ahmed, 2002).



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